Abstract
Sperm usage was investigated in a naturally mated honey bee queen. We collected worker progeny arising from eggs that were laid sequentially during three sampling periods. Paternity was determined by analysis of three polymorphic microsatellite loci, leading to the conclusion that the queen had mated with seven males. Direct analysis of the sperm from the spermatheca revealed no evidence that sperm from additional males was present inside the spermatheca. Frequencies of different subfamilies differed significantly and ranged from 3.8% to 27.3%. In the short term, the frequencies of subfamilies among the eggs laid did not change over time. The frequency of eggs of a particular subfamily was statistically independent of the previous egg's subfamily. Thus, there is no evidence for non-random fine-scale sperm usage, and we estimate the effect of sperm clumping to be less than 6%. We conclude that the sperm is mixed completely inside the queen's spermatheca. Our results suggest that taking brood samples from comb cells next to each other is a statistically correct way of independent sampling of subfamilies at a given time in honey bee colonies. Furthermore, any bias in subfamily frequencies in offspring queens due to sperm usage can be excluded. However, the analyses of progeny samples taken 12 months apart do not allow us to exclude moderate fluctuations of subfamily frequencies in the long-term.
http://link.springer.com/article/10.1007/s002650050436
Abstract: A honey bee queen mates with a number of drones a few days after she emerges
as an adult. Spermatozoa of different drones are stored in her spermatheca and used for the
UHVWRIWKHTXHHQ¶s life to fertilize eggs. Sperm usage is thought to be random, so that the
patriline distribution within a honey bee colony would remain constant over time. In this
study we assigned the progeny of a naturally mated honey bee queen to patrilines using
micrRVDWHOOLWH PDUNHUV DW WKH TXHHQ¶s age of two, three and four years. No significant
changes in patriline distribution occurred within each of two foraging seasons, with
samples taken one and five months apart, respectively. Overall and pair-wise comparisons
between the three analyzed years reached significant levels. Over the three-year period we
found a trend for patrilines to become more equally represented with time. It is important
to note that this study was performed with a single queen, and thus individual and
population variation in sperm usage patterns must be assessed. We discuss long-term
changes in patriline composition due tR PL[LQJ SURFHVVHV LQ WKH TXHHQ¶s spermatheca,
following incompOHWHPL[LQJRIGLIIHUHQWGURQHV¶ sperm after mating.
Does Patriline Composition Change over a Honey Bee Queen’s Lifetime? (PDF Download Available). Available from:
https://www.researchgate.net/public...tion_Change_over_a_Honey_Bee_Queen's_Lifetime [accessed May 21, 2016].