Hi
you mean different Apis mellifera types (subspecies) don't you, say 50% A m carnica and 50% A m mellifera?
That could prove to be very difficult, you would have to make sure the carnica which build up earlier (according to Ruttner) do not have more Drones in their hives, also there's always the question of where did the virgins fly to, ...someone may say that amm virgins performed Near Apiary Vicinity mating ... and other such things.
The idea had crossed my mind as well, even with the possible counter arguments the results of the experiment would add the findings of a third study, with this suggested Isolated Mating Study, I think being the most valuable.
BUT
would a more extreme version not be better.
IF we took, say 10 Amm virgins and released them on an island (isolated mating station) with NO Amm drones, say carnica drones only, and also released carnica virgins (just to prove that the island provided the necessary mating conditions, such as weather, etc.), we could provide strong evidence as to whether Amm virgins mate with non-Amm or not. I think that might actually be do-able, do you think this would be a good idea?