I use bare hands or nitrile gloves. With larger agressive colonies I prefer to wear gloves, for smaller ones and nucs just bare hands suffice.
Colonies don't "change on the drop of a pin", you can judge the bees reaction by the time you crack the crownboard, and furthermore you can also tell if they start bubbling up.
If the colony is a evil one, I'd double glove but thats incredibly infrequent, just once every few years and allows me to get in and sort the queen.
The issues described can often be cured by ruthless culling of the queens of colonies with bad temper. However I'm continually amazed at the lack of willingless to cull bad queens - they simply soldier on with bad temper. Or worse, they cease inspections - which just leaves the colony to keep launching drones into the local area.
Adam