I have teached in several forums how to use trickling. This is from Finnish forum
http://bee.freesuperhost.com/cgi-bin/yabb2/YaBB.pl?num=1136437131
These are days when I wrote on Beesourceforum 6 years ago too
Trickling of varroa
05.01.06 at 06:58:51 Cure is given before winter when brood is away and bees are in winter ball. +5 - +10C (41-50F) is good temperature. Bees may fly back to hive at this temperature but even colder is good.
Brood area contains mites. It it best to destroy them if it is small. If bees do not fly and you put frame out, birds will pick brood from frame. If you leave brood you may have hundreds of mites there.
Get a vaccination syringe from drugstore so you see ecaxtly needed measure to spray.
You may put paper on bottom boad before handling. You will se how much you get mites when you take it away.
Recipe:
Take 7,5 gr oxalic acetic powder and put it into 0,1 liter warm water.
Take 100 gr sugar and dilute it in the solution.
(Euro coin is just 7,5 g. You can weight your coin and you can use as weight. Own balance is easy to do. Our coin weights are in internet. There are cheap digital kitchen balances in stores which have 1 g accuracy. )
This 160 ml volume is enough for 3-5 hives, depending of the amount of bees .
Give droplets together 4 ml solution in one gap of frames, which is full of bees from edge to edge.
Do not give for one box hive more than 40 ml, and this only for hive which is totally full of bees.
Do not give for 2 box hive more than 50 ml.
DON'T GIVE TWICE HANDLING. It is harmfull.
Sweden:
http://www.algonet.se/~beeman/research/oxalic/oxalic-0-nf.htm
IT TAKES ABOUT 2 WEEKS BEFORE YOU SE FINAL RESULT. HALF OF MITES HAVE DROP AFTEN 7 DAYS.
.
Finnish beekeeping specialist Ari Seppälä calculated from Finnish
varroa trickling research following lag time :
After
cure ---- rate of all falled
1 week ---- 54 %
2 week ---- 20 %
3 week -----13 %
4 week ------8 %
5 week ------5 %
A finnish researcher Seppo Korpela has calculated during many years mites' drop
year 2000 altogether 34468 falled mites
1-2 weeks 96%
3-4. weeks 2,4 %
v. 2004 ... 10730 mites:
1-2 weeks 98 %
3-4 weeks 2 %
Y 2005.... 12270 mites
1-2 weeks 96 %
3-4 weeks 4 %