- Joined
- Jan 13, 2015
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- Location
- Bedfordshire, England
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- Langstroth
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- Quite a few
The same argument that Gilberdyke John made still holds though. You could argue that the damaged area where the section of brood comb was cut out is also a signal to the bees that something is wrong with the brood.
Personally, I use the pin-killed brood method and have no problems. The important thing to note is that the brood should be of the right age. Too young and you can miss the larva when you pierce the capping, and, too old and there is too much resistence to the insect pin. The pupa should be at the pink eyed stage. You may, therefore need to mark a specific comb for use in the test or manually uncap some cells to find pupae of the correct age
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