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Thanks. I'm fairly resigned to losing this colony but have five other nucs over wintering that had the same treatments so was thinking a spring treatment would be useful for those. Sorry didn't make that clear .
Won't use apistan again lesson learned.
I'll refer you to this relatively recent discussion..... @Boston Bees is the sage one on this. That thread also shows that you need to choose carefully from whom you take advice:
https://beekeepingforum.co.uk/threads/supers-and-varroa-treatment.51424/#post-787559
The makers of the product do accept that there is a problem with resistance, but they also offer what sounds like constructive advice on how to manoevre around this:
https://www.vita-europe.com/beehealth/products/apistan/#delay