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The above is suitable for 2 colonies, not 5. If on inspection when you come to do the treatment you find the colonies are weak it might go a bit further but you need to plan on 50mls per colony. But the point about making up a large amount is the errors from weighing are reduced. My comment about being a gram or so out applies to large amounts. A gram out from 7.5g is an error of over 10% but a gram out from 37.5g is an error of less than 3%. It really isn't difficult folks. The stuff is cheap so make plenty so you get the proportions correct.
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