Trickling method

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Queen Bee
Nov 8, 2008
Reaction score
Finland, Helsinki
Hive Type

Posted 8 Dec 06

Oxalic Acid Trickling Method

By David Ashton

Ref: Danish Beekeepers Associations website

( see also comments at Nosema Ceranae & Denmark )

The late season varroa – control is important. The treatment should take place when autumn feeding has finished, and the colony is free of brood.

Oxalic Acid trickling

Oxalic acid is an organic acid, which is found naturally in honey. The method is extremely effective, very quick and inexpensive


For varroa control the Oxalic acid is used in a very weak solution (3.2%). This solution is a very weak corrosive. But you must be very careful to avoid contact with the skin as oxalic acid can be absorbed through the skin. During treatment it is important to use acid resistant rubber gloves. Whilst mixing the solution you should use, gloves, safety goggles, and respirator (P2 Dust Mask).

Treatment in Brood Free Period

‘Oxalic Acid trickling’, does not work behind the brood cells sealed cap, this is the reason why treatment should take place in periods when none or very little brood as possible is present. We would normally recommend a treatment in late October - November (but account should be taken of climate for example in western seaboard of British Isles the gulf stream keeps the temperature up so brood rearing goes on into December) As the climate warms its important to check if brood rearing has stopped, so it could well be that treatment should take place in November or December. The important thing however is that the beekeeper must not fall in to the trap of forgetting to use an oxalic acid treatment. Bees, which for various different reasons go into winter and hibernation with too many mites, will suffer a large amount of damage and harm. One should there for in your considerations consider what is said. “ Treatment when brood free, so that those mite present can do the least amount of damage ” In other countries outside of Denmark, there is a tradition because of climate to give a much latter treatment!

It has previously been said that the colonies should be treated so the bees will have a couple of flying days after treatment. There is however no research that shows this to be necessary

Mixing the Solution

For the treatment then use oxalic acid – bi hydrate

The Oxalic Acid is mixed in proportions 1 litre distilled water: 1 kg sugar: 75 g oxalic acid – bi hydrate. This is enough for 55 colonies. You need to adjust this in proportion and/or use the Danish beekeepers calculator below.

You need to be aware that mixing very small amounts for example just two colonies for example, can create problems, as ordinary kitchen scales can give the wrong mixture. Consider weighing a minimum of 0.5 litre distilled water: 0.5 kg sugar: 37.5 grams oxalic acid – bi hydrate.

Durability (Shelf Life)

When we only recommend autumn treatments, there is no reason to keep your surplus mixture; it is best put down the drain. If however you wish to keep your mixture, we should draw attention to the fact that it will keep up to six months in the dark in the cold of a cellar. The mixture should also be kept in child safe and secure bottle.

How to use it?

The Oxalic Acid mixture should be luke warm. You should use a 50 ml dosing syringe. You trickle 3- 3.5 ml per frame space with bees. That is to say 35 ml per colony. This is a very small quantity so it’s a good idea first of all to practise with ordinary water. It is important that the trickling takes place direct onto the bees and not just on the frame tops were the bees would let it lie.


The outdoor temperature above 0 degree C

Damage to bees

If the Oxalic Acid is used correctly, in the correct dosage there is no risk to the bees or the risk is very small. If you over dose then you can loose a lot of bees and weaken the colony.

How often should you treat?

Danish and international research has shown that if bees are treated more than once per generation, it can result in damage to the bees and a reduction in the strength of the colony. We recommend there for that you should only treat the bees once each season. The best time to treat is in the autumn early winter when the colony is brood free. We have however with large-scale beekeepers treated with success both in the autumn and in the spring with Oxalic Acid Trickling Method.
If you make for 3-5 hives, make

100 g warm water + 100 g sugar + 7 g oxalic acid.

40 ml to one box hive and

50 ml to 2 box hive.

You need not lift the upper box when you trickle 2 box.
Trickle Method

Hi Finman,thanks for the info I have noted dosing level of 3-3.5ml/seam of bees is the 5ml/seam that is often quoted too much or just not necessary?
Hi Finman,thanks for the info I have noted dosing level of 3-3.5ml/seam of bees is the 5ml/seam that is often quoted too much or just not necessary?

Do you have bees clustered in the outer frames?

I do 5ml per seam over max of 8 seams = 40ml per box.
Hi Finman,thanks for the info I have noted dosing level of 3-3.5ml/seam of bees is the 5ml/seam that is often quoted too much or just not necessary?

The dosing level has been reaseached carefully during years by experts. If you now change it, you take a risk that it does not work.

When I have 40 ml in my syringe, I rible lightly all seams first and the rest I put here and there. Bees spread the sticky stuff to each other.

If you put double dosage, bees become wet.

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