Should? Based on what?
Wasn't talking about sterilization. Disinfection is something entirely different. And the reason why disinfection is required is because the principal source of pathogens that will be found in the 'excluded compartment' of the Flow frame that isn't 'part of the hive' will be the spores found in the honey that are likely to germinate once water of activation levels become favourable. Washing and drying the frames immediately after use will potentially reduce the need for disinfection. Leave it for eight hours under certain conditions and disinfection will become a must.
No they won't because by definition they're not accessible and they are made from material(s) foreign to the ones normally found in a hive and no-one yet knows what impact that may have on the nature and type of organisms that may grow as a consequence.
http://thirdworld.nl/microbiology-and-food-borne-pathogens-in-honey
It's not the difficult question it's the fact that you seek arguments to highjack the OPs thread. So if you want to ask the question then show a little respect and ask it in a new thread.