I'd be interested in hearing how many colonies B+ currently is managing and what he feels is the source of their long term survival.
Like most beekeepers, the number of colonies I manage goes up and down throughout the season. I say on my profile 70+ and that is a pretty good guide.
I have over 30 daughters of 6-1-1037-2015 along with other groups of daughters which I regard as worth breeding from. I also have older test colonies (pure island mated carnica) which I am keeping for one reason or another.
As far as 2016 test colonies goes, I have the following:
VSH Line (Island mated on Vlieland)
55-2-70-2016 = 55-2-36-2016 * 18-26-7268-2013
55-2-73-2016 = 55-2-38-2016 * 18-26-7268-2013
55-2-77-2016 = 55-2-40-2016 * 18-26-7268-2013
NL Line (Instrumentally Inseminated)
55-9-90-2016 = 55-3-44-2014 * 18-26-7268-2013
55-9-89-2016 = 55-3-44-2014 * 18-26-7268-2013
I.B. Celle Line (Island mated on Neuwerk)
6-1-585-2016 = 6-1-1986-2014 * 6-1-0563-2012 Line A1-013
6-1-608-2016 = 6-1-1986-2014 * 6-1-0563-2012
6-1-628-2016 = 6-1-1986-2014 * 6-1-0563-2012
6-1-640-2016 = 6-1-0381-2014 * 6-1-0563-2012 Line A2-001
6-1-645-2016 = 6-1-0381-2014 * 6-1-0563-2012
6-1-649-2016 = 6-1-0381-2014 * 6-1-0563-2012
6-1-773-2016 = 6-1-0381-2014 * 6-1-0563-2012
6-1-703-2016 = 6-1-1915-2014 * 6-1-0563-2012 Line A3-066
6-1-727-2016 = 6-1-1915-2014 * 6-1-0563-2012
6-1-734-2016 = 6-1-1915-2014 * 6-1-0563-2012
all of these are pure carniolans and could be considered "breeder queens" in their own right without further testing. In BeeBreed, we only breed from those which perform better than the mean for that family group though so it is important to test full-sibs in different conditions.
Their long term survival stems from the fact that I can select the best performing stock that I have and mate/inseminate it with semen from the best performing stock in the breeding population (many thousands of queens are tested each year). I am not working alone.