Mo, Could you please give the full method you use:Sorry MM,
I should look and inwardly digest who has posted etc.
The staing ing of pollen grains is normally done using safranin stain ready mixed with a Glycerol base. All that happens is you place the pollen grains on a smear of glycerin (stained), and cover with a mountant I use practermount, and place cover glass over using a pair of tweezers to lower it down.
The colour that shows on the exine of the pollen grain is stained by the safranin penetrating the hard exterior shell. I have experimented with a number of different colours and methods but with little success. You might try a blue or Orange and leave the grains soaking for 10 15 Min's. before washing off.
If you are taking the BBKA microscopy exam then try to get hold of a booklet named Pollen , its collection and Preparation for the Microscope. by John White (Second Edition)
If you have difficulty and do not have Module 9 Microscopy Study Notes then I could lend you them for a short time.
All the best to you all and sorry for the sharp reply earlier.
the pollen sacs are made up of many pollens, it is impossible to tell what plant is has come from, we select a plant before the flower opens and keep it indoors, when it flowers we can take the pollen sample and can be sure it’s an accurate sample.2nd dumb post of the morning -
Why is the colour of pollen different from that which the ladies carry back, eg OSR is shown as being purple?