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Crocus Pollen

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wilderness 

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beginning to get the hang of this microscopy lark !!

I use a free program called Picolay which gives you an image with a greater depth of field. You take photo slices at various focus heights and the program adds them all together.
 

Onge 

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Or is it just fish eggs (only joking).

Great photo. :)
 

Bcrazy 

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Great photo but I would prefer it to show individual grains instead of a clump of grains, another thing it does not show any detail of the individual grains.
I have not come across Picolay but if it produces photo's like that its worth using.

Microscopy is a different hobby and the field of microscopy is massive.

Regards;
 

wilderness 

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Hi BCrazy,

I'll see what I can do to separate the pollen grains. I can see detail on the surface of the grains when looking through the 'scope by eye but the electronics blur the detail.

This is a Bresser Biolux which I got from Lidl for £50 so I'm pleased with what it does.
 

Moonglow 

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I am glad you have told me that it was the Lidl scope, I had one for christmas and on seeing your images,was wondering if I could produce images like yours, as they are impressive
Could you please let me have a link for the free picolay?
Brilliant results
 

wilderness 

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link to Picolay http://www.picolay.de/
There is another program which is supposed to do the same thing but haven't tried it. http://www.hadleyweb.pwp.blueyonder.co.uk/CZP/News.htm

I don't use the software that came with the Lidl microscope as I found it very slow to react to changes in focus. That may be down to my old desktop not having sufficient RAM or the latest video drivers. The program I use is called Vision GS from here http://visiongs.en.softonic.com/.

Although it is for webcams it works OK for the microscope cam.
 

Bcrazy 

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Just thought I'd show you Crocus pollen

Regards;
 

admin 

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Crocus is my favorite pollen to see going in to a hive,I love the gold specks it has in it.
 

thada1 

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I used a similar program (Zerene Stacker) to build these images of an anther and the stigma of a purple crocus. The first two were done with a Brunel DM2 microscope and my Canon 40D, the larger FOV was done with a 50mm lens and extension tube for close focusing.
 
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