Finman : my chalk brood went away several years ago and haven't seen it since in my colonies. I especially look out for it as I need examples of chalk brood to show beginner beekeeping students so now need to beg chalk brood mummies off others.
The role of CO2 in chalk brood spore activation was discovered way back in 1987
Journal of Apicultural Research Vol. 26 (4) pp. 243-246 December 1987
Carbon dioxide activation of spores of the chalkbrood fungus Ascosphaera apis
L. A. F. Heath And Barbara M. Gaze
Abstract : Carbon dioxide has been shown to be important for the activation of spores of A. apis. About half the spores under test were activated at less than 5% CO2 in air, while at 12.5% CO2 virtually all spores were activated. Ten minutes' exposure to 10% CO2 in air was sufficient to initiate activation. Larval respiration produces CO2, and thus larvae must initiate the activation of spores with which they come into contact.