Don't forget that the number of mites on the vertical axis of the B+ chart is not the drop for the whole colony - it is the number of mites found after sugar rolling 10g of bees. If you're counting natural drop of a colony on a monitoring board, you'll probably have to use calculator or chart 54 on page 33 in
Think about what is happening and when you would perform these tests:
The natural mite drop is performed early in the spring when you wouldn't want to open up a colony. It is probably going to be too cold and you don't want to risk chilling any young larvae the colony may have. It measures phoretic mites that are naturally dying or being groomed off the workers. They fall through the open mesh floor and are collected on a board under the OMF. If you don't have something sticky on the board, they may become the meal of any microscopic creatures (or even ants, etc) that live under the colony. You are only interested in the dark red mites which are the adult females as these could have laid eggs which would become the next generation of mites. You are not interested in lighter coloured mites (immature females or males) as they cannot survive outside of the cell.The purpose of this test is only to give you a number which represents the initial infestation of the colony and other tests are better later in the season.
As the weather improves, and the colony expands, you may wish to monitor the growth in the population. This can be done with various tests (i.e. sugar roll, soapy water wash, etc). They are best done with adult workers from the supers to avoid the risk of killing the queen by accident. Also, there is evidence to show that the population is more uniform (consistent for measuring purposes) in the supers furthest away from the brood nest. This may seem somewhat counter-intuitive because varroa mites prefer to parasitize young nurse bees because they actively feed the young brood in cells that the varroa wants to reproduce in. However, bees will fly away from the colony and reduce the infestation in the colony. By taking them from the supers, you are also smoothing out any peaks and troughs caused by mites emerging from the cells at a certain point in time. Effectively, its an average...not an exact number, as this will change all of the time.
Other tests may include pin-killed or freeze-killed (with liquid nitrogen) brood, but, this shouldn't be confused with varroa monitoring. These tests are concerned with the rate at which worker bees are able to detect and remove dead pupa. They test "hygienic" behavior. I perform these tests during the first week of July but follow them up with repeats about 3 weeks later. The hygiene will be expressed as a percentage of the number of cells completely emptied within a given period (8-12 hrs for pin-killed brood and 24 hours for frozen brood).
Colonies which express the highest hygiene percentage can be tested further (i.e. hygiene is considered a pre-selection test for VSH testing because of the amount of effort involved in these tests). I intend to begin VSH testing for the first time this August as I now have stock that should express high VSH. Basically, this involves uncapping 30 pupae (at least 7 days post capping -
http://www.beekeepingforum.co.uk/album.php?albumid=751&pictureid=3789) and counting the number of reproducing / non-reproducing mites in the cells. VSH is also expressed as a percentage.