Just resurrecting this thread from last autumn, where I posted asking for advice on a possible Queenless colony. As related in the first post I hadn't been able to find any eggs or brood for around six weeks or so after removing the last api-life treatment, and also hadn't been able to find a Queen despite several quite careful searches (although I'm pretty rubbish at finding Queens and this one wasn't marked).
Anyway, as others have been reporting, my hives are very lively today including this one. They seem quite purposeful and are bringing in pollen. Is this a good sign (ie might they be bringing in pollen anyway if there was no Queen)?