LASI found that even the highest doses (well in excess of 2.2g) had no ill effect on the bees, using syrup as a delivery method (as in trickling) could have a negative effect as the bees ingested much of the OA as they were cleaning the syrup off themselves - no big deal for a worker bee with her limited lifespan, but a queen, subjected to multiple doses it's a different matter which is where the 'once a year' recommendation for all oxy delivery methods (api bioxial, oxybee et al) came into effect. Another example of not letting common sense and logic get in the way.