failing Q spermatheca test

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wightbees

Queen Bee
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Something i read which may be of use


There are times when a queen bee is failing in some way that it becomes necessary to perform the unpleasant task of killing her. Most beekeepers feel a pang of compassion for the queen as they pinch her head and also a sense of economic loss for the money they spent to buy her. There is however a very simple test that can be performed on her dead body in a few seconds, that can at least provide some valuable information to beekeepers, so that the process does not have to be a complete waste. The technique is surprisingly easy and can be performed in the field with bare hands.

When the queen is mated (by 10-20 drones) the sperm is stored for her lifetime in an organ called the spermatheca. By removing and examining the small, liquid-filled sphere one can tell how much sperm was available to the queen at the time of her death. You may ask what good this information does you now that the queen is dead. Well, by confirming or denying your suspicions about her, you educate yourself over time as to the appearance of queens in different conditions and so make more accurate assessments.

This technique is especially important for queen breeders, queen producers and people who cage the queens for sale, by improving their culling accuracy

Spermatheca from a well mated queen is tan colored

Depleted spermatheca is translucent

Spermatheca from a virgin queen is clear


Instructions

Kill the queen by pinching her head.
Grasp the last two abdominal segments (where the stinger is) with either your fingers or forceps and pull this segment away from the rest of the queen. Make sure you grab just the last two segments, and don't worry about being stung.
Discard the queen's body but keep the last two segments..
The spermatheca is buried in the mess, but is remarkably sturdy and can be squeezed out by rolling your fingers together, or between the backs of your thumbnails.
The spermatheca rolls out as a perfectly round sphere about 1 mm in diameter. It appears white because it is covered with a tracheal net to provide oxygen to the sperm. This covering is removed by rolling it for a few seconds between your fingers. Now it is ready to analyze.
1) If the spermatheca is perfectly clear, the queen has not been mated. She was a virgin queen.

2) If it is tan and opaque there was plenty of sperm to fertilize eggs. This was a well mated queen.

3) If it is milky and translucent, the supply of sperm was running low, either from age or inadequate mating.

It may take a couple examples to distinguish the tan / opaque condition from the milky / translucent appearance.



What to do with this information.

Once you determine the state of the queen's spermatheca, go back and take another look at the brood pattern, egg placement, condition of hive, queen cells, how did the queen look, how did she act?

The next time you see these signs you'll have a better idea of what condition the queen is in. More information makes for better decisions. With a little practice this technique will prove to be a valuable tool.

If the queen was newly purchased, and the spermatheca was not opaque, therefore not fully mated, let your queen producer know this information. This feedback is important so queen breeders can remedy the situation by providing more drones. Weather conditions, such as cloudiness or temperatures less than 69 degrees during mating periods can contribute to low sperm count. We can't change the weather but we can add drones colonies to give the best chance for success.
 
Your enforced inactivity has produced some gems, Where did you find the above? I squished a DLQ last year and could have used the above to confirm had I known - Many thanks

Mike
 
Your enforced inactivity has produced some gems, Where did you find the above? I squished a DLQ last year and could have used the above to confirm had I known - Many thanks

Mike

I'll second that
 
wightbees

very interesting : i think you might be a proper beekeeper now :party:
 
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I know Ph thinks there might be hope for me somewhere :D
 
.
I wonder what the bee breeder can do even if he knows that spermatecha is not full?
I rear my queen my self. I keep spare queens and change the queen if it is not good.
 
The only use I can see is if you buy a queen, and she turns out to be a drone layer, or improperly mated then you go for the test and then moan to the supplier.

Which I would do anyway with out messing about with a PM. Life is too short.

PH
 
I hope some of it is,i have been trying hard this week to learn as much as i can about
genetics and hygiene of bees.I have learnt how to look at capping's to see if they have been recapped or just slightly recapped.This according to some is a way of telling if you have bees that would fit into a selecting breeding for VHS bees.
 
What ever VHS stands for.

Not taking the micky here but with 4 colonies you are not in a position to breed.

Nor am I with my numbers.

200+ there is enough material to possibly make a start.

One has to be realistic.

PH
 
maybe, but to learn is a gift of which you need nothing.
 
Freeze your specimens and buy a cheap second hand micropscope, it is amazing what they have packed into this tiny little insect.
 

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