I suspect they had a virgin, or, you had a large number of older foragers in the nuc. If you follow the method I described above, you'll have more success.
All usual precautions taken and usual supects excluded.
Colony was an active double nat so not a nuc.
Before introduction of 2 & 3 rd queen a test frame showed QC's. So unlikely there was a resident Q, virgin or otherwise.
If you do enough re-queening you will find the odd one that doesn't play the game.