No, there are choices.
We can normally do it, from scheduling necessity, pretty well within the first few hours from making up a nuc, but preferably make it up and leave it queenless for a week or so. Then destroy any cells....and you need sharp eyes at times for late started cells that are merely the slightest of changes at the cell mouth and an increased supply of royal jelly........and can also be on drone (the bees don't know they are trying to raise a dud at that stage).
Same applies to full size splits etc even full hives.
Then find a patch of hatching brood, remove ALL hive bees from it and press the cage into the brood sealed all round. We use the white Nicot ones so from here on what I say might need to be adapted to other makes or home made.
Before pressing fill the candy tube with a nice stiff fondant....add icing sugar if you have to and work it well in......remembering to remove the little plastic barrier if its the first time of using the cage.
Place the queen cage in the water tub and make sure the bees are thoroughly wet. remove the plug from the cage. then open queen cage and pick out the queen only. place her in through the hole in the press in cage and keep your finger over the end until you see her walking away from the hole. then replace the plug. Put the frame into the hive with another frame of brood against the cage. Hive back together and give it a feed of syrup (simulates a nectar flow and radically improves acceptance). Do NOT get tempted to go nosing in before a week has passed.......every day before that increases failure rate.
As the brood hatches the queen then has her own little crowd of companions and can even start to lay even before she has been released from the cage.
Its not complex but if any questions don't worry about asking.