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Here is another good document from Australia 1996
PROTEIN CONTENT AND AMINO ACID PROFILES
OF HONEYBEE-COLLECTED POLLENS
http://www.honeybee.com.au/Library/Pollenindex.html
Protein and Amino-acids
Protein is not just protein. Protein is made up of separate parts called amino-acids.
Building up body muscle with protein is a bit like building a house. The protein can be likened to the "building materials" as a whole. The amino-acids are like the individual kinds of building materials, such as timber studs, bricks, window glass, nails and roofing iron. However, in the case of protein, most amino-acids can be reconstituted from other amino-acids.
In digesting the protein, the animal can manufacture some of the required amino-acids from other amino-acids that may be surplus.
This is like a builder using a surplus stud for making noggins or a door frame, or some flooring for a brace, or rafters for floor joists. There is a degree of flexibility in how building materials may be used.
However, there are some specific building materials, such as nails or glass or roofing iron or tiles, that cannot be reconstituted from other items.
Not only are these essential building materials needed, they are needed in specific quantities in order to construct the building according to the plans.
It is the same with protein and amino-acids. Some amino-acids cannot be reconstituted from other amino-acids. These amino-acids are called essential amino-acids.
Essential Amino-acids
Some amino-acids are an essential part of the protein digested by the bees, while others are reconstituted from surplus non-essential amino-acids. Essential amino-acids are required in definite proportions of the protein digested as protein.
In 1953 Dr. A. De Groot studied the amino-acid and protein requirements of honey bees and found that they need ten (10) essential amino-acids, at levels ranging from 1% to 4.5% of the protein digested. These results are listed in Table 1.
Table 1: Essential amino-acids for honey bees
Amino-acid Minimum required % of amino-acid in protein digested
Threonine 3.0
Valine 4.0
Methionine 1.5
Leucine 4.5
Iso-leucine 4.0
Phenylalanine 2.5
Lysine 3.0
Histidine 1.5
Arginine 3.0
Tryptophan 1.0
If one of these essential amino-acids is not present in the amount required by the bees, then the bees cannot fully digest as protein all the protein they have eaten. For instance, if one of the amino-acids is required at 4% and is only available at 3%, then only three-quarters of the total protein consumed can be utilised by the bees, as body-building protein.
Analysis of pollen protein has indicated that most of these essential amino-acids are at satisfactory levels. However, Iso-leucine and on a few occasions Valine are below these desired levels.
NOTE Tryptophan is not been found in pollen anylysis , because it does not emerge out. However it is enough in all pollens.