BeeBeeKa
House Bee
- Joined
- Mar 4, 2014
- Messages
- 282
- Reaction score
- 2
- Location
- Co. Sligo, Ireland
- Hive Type
- Langstroth
- Number of Hives
- 8 poly hives
I.e. I found this article, mentioned Yucel and Dogaroglu, interesting :Banu Yukel of Eig university and and Mohsin Dogaroglu ot the University of Thrace (both in Turkey) Based on field tests and compared to controls testing both fumagilin anf Thymol over successive years - there are others as well
http://scientificbeekeeping.com/the-nosema-twins-part-5-alternative-treatments/
“A promising alternative that really catches my attention is good old thymol—which is widely used for varroa treatment, and as a disinfectant and fungicide. Braga, et al (2007) found that its mode of action against the yeast Candida was to affect the “architecture of the [fungal] envelope,” thus hampering its infectivity. Well, it apparently also may control nosema when used in the form of thymolated syrup (doesn’t that term just roll off the tongue nicely?). I came across a reference to it in a state newsletter, which had picked it up from another, and yet another, and finally got to the original paper by Rice (2001) from Australia. What had piqued my curiosity was that all the references gave the recommended concentration as 0.44mM, which is a rather precise chemical term that no beekeeper would ever use. Rice apparently back-calculated that specific concentration from a recipe used by a beekeeper named Brown, who likely got it from another, Manley (1946, which I downloaded from the Australian library), who got it from a long letter from a Dr. Killick, who thought that the addition of thymol would make sugar syrup convert to a more natural “honey”—nosema wasn’t even mentioned. Rice then tested thymol against nosema, but used analogous lab surrogates–the Corn Earworm infected by Nosema vespula, which infects European wasps. For some reason, he also used weaker concentrations than 0.44mM—0.15 and 0.30mM. Anyway, he found that thymol killed N. vespula in caterpillars. He also discusses possible modes of action of thymol against nosema. It appears to me that the 0.44mM strength is based upon an old arbitrary concentration intended for another purpose, that serendipitously happened to also be effective against nosema in Australia.
Well folks, Rice’s was a great study, but I really couldn’t call it a slam dunk for our purposes. However, Yucel and Dogaroglu (2005), in Turkey, performed a controlled trial comparing thymol against fumagillin. The authors fed infected colonies 150ml of light (30%) syrup at weekly intervals for 4 weeks (for a total of 600ml, or approximately 2½ cups), both spring and fall. The treatment syrups contained either thymol at 0.44mM (they must’ve read Rice), or Fumidil-B at 1 gram per feeding (this is approximately the label rate for the small colonies they studied, but in less syrup); the controls were plain syrup. The treatments were continued for 3 years, in order to cleanse the colonies of residual nosema spores (the authors state that the infection was by N. apis, but had no reason to suspect that the colonies could actually have been additionally infected by N. ceranae).
The results were impressive for thymol! Over the term of the experiment, both fumagillin and thymol decreased colony losses, and increased number of bees, amount of brood, and honey yield, but thymol did the better job in every parameter measured! Plus, the thymolated colonies had fewer winter losses. Note that the study used only a small amount of very light syrup. The authors hypothesized that the syrup would be concentrated by the bees, thus effecting a stronger concentration of thymol once processed by the bees. Also note that it took three years of either treatment to really get nosema spore counts down!
With great hopes I fed several hundred gallons of thymolated syrup to my own infected colonies. Unfortunately, my results do not support the efficacy of thymol, nor do other short trials that I have seen from Europe and Canada. I am currently testing it at 3x strength. It may yet prove to be effective in the long term, but I’d consider it experimental for the time being.”
There is a lot of studies indicating failure of fumagilin to cope with nosema novadays. I.e.” Nosema ceranae Escapes Fumagillin Control in Honey Bees” : http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003185
(See a list of references in there too).
Thus I`m on a side of thymolated syrup in regard of nosema so far.