Nothing wrong with counting if you have the time and inclination. However you do have to understand the limitations. The calculators on the NBU website and elsewhere based on mite drop are for the natural rate of mite death. That is, a population of mites will breed and die at a predictable rate. The assumptions are that the age spread of the mites is even and you see all the dead mites. What can happen is mites get lodged in empty cells, blow off the board, are taken by ants, have ages bunched because of previous brood breaks and lots of other factors. So the calculation is not precise, but a reasonable guess the hive has at least that many mites. Statistically, you get more reliable results if you use periods of several days, and even better if samples from more than one period produce similar results. If the time of year allows drone uncapping that can be useful confirmation, as can sugar rolling.
When you use treatments to kill mites, that's not the natural death rate. One day doesn't mean a great deal. It's the count over a whole treatment period that matters. Mites will die as they emerge from the cells and are exposed to the treatment, which is why most treatments are at least a full mite life cycle, extending to two. As a percentage kill rate, it's the number dead over what you started with as a percentage. But you cannot know the starting numbers precisely. Researchers can estimate the mites after treatment using an additional, different treatment with chemicals not available publicly, lethal rolling or even killing the entire colony. Non of the methods are helpful to a productive colony.
After treatment, the more bee friendly methods of estimating are even more problematic than before because of brood breaks, few drones to uncap, mites arriving from other colonies and the general reduction of brooding going into autumn. The methods are not going to show a clear difference between, say, 80 and 90% effective. Trying to estimate before and then a month later, my hives have drop results with thymol treatments that suggest effectiveness ranging from 50 to 90 percent, and that's probably related to weather and the state of the colony being treated. During treatment, if no mites dropped, they probably didn't need treatment but that's hard to say in advance. If 1000 mites drop then at least the infection is less than it was, but expect to need further treatment later such as oxalic over winter.[/QUOTE
Very informative reply! Useful to all.