Due to emergency circumstances, I had to introduced a mated queen using a push in cage to a handful of bees (less than 50 bees) made queenless for about 5 hours, with plenty of stores & drawn combs, in a poly nuc. The following day (yesterday) I made queenless for 9.5 hours 2 capped brood frames & adherent bees from yet another colony. I then Introduced that into the poly nuc last night at 11:30pm - the frames and bees went peacefully into the nuc, no fight whatsoever as if they were always from the same colony. The two colonies were actually sort of clustering in their respective nucs, and when I opened them to unite them no bee attempted to fly, except for a dozen of them who were attracted to the lamp like moths. After watching them for a while I released the queen and she was straight away tended by them. Question, please: when can I open that nuc entrance? I want to make sure they are all impregnated with their new queen pheromone and come back to that nuc after go foraging because one of them came from a colony that is only 3 minutes drive away and another one (the one I got only less than 50 bees) came from a hive next door to where the nuc now is (about 1 metre away, and the nuc is facing a wall, 180 degrees opposite direction to the entrance of the nuc they came from 2 days ago and that hive is facing the opposite wall). Thanks in advance