Hi I've managed to find a copy of the researcher's PhD, at University of Sussex (Dr Hasan Al Toufailia). You can find it on the University of Sussex website under that particular researcher's details, but see Chapter 6 pages 54-59 for the twice treatment by sublimation experiments, results and discussion. Some other v interesting research on varroa resistance etc featured in his doctorate. May be of interest to
@Plastics &
@pargyle too.
The key paragraphs not included in the extract, I've copied below. They highlight a twice treatment to a brood less colony in winter is effective as an annual treatment.
Happy Bedtime reading!
Elaine
http://sro.sussex.ac.uk/id/eprint/62056/1/Al Toufailia, Hasan.pdf
Extract from key paragraphs below:
"Although the second treatment of OA was less effective than the first the benefits to beekeepers of double OA treatment probably outweigh the costs given that OA is cheap and quick to apply via sublimation and causes no harm to the bees or the colony (this study; Al Toufailia et al, 2016). The main advantage of double treatment is that it will reduce the varroa population to such a low level that it should take at least one year to build up to harmful levels. As a result, annual double treatment with OA in broodless hives may be sufficient to provide full varroa control. The second OA treatment, which gave only 87.2% mortality, will still result in the varroa population requiring approximately 3 additional doublings to reach its original level, and 8 population doublings for both treatments combined. Previous research has shown that a single winter treatment of broodless hives with oxalic acid via sublimation is insufficient to prevent many non-hygienic colonies from showing overt symptoms of deformed wing virus, a pathogen that is vectored by varroa (Al Toufailia et al, 2014). A further advantage of double OA application is that it could act as insurance in the event that a single application is inadvertently made incorrectly and so kills many fewer varroa than expected"