we always mark and clip our queens, but last week we marked all of this years queens, all went well except the last one which was a very large colony taken from a split and the queen had been very hard to find so we were determined to mark her even though the time was getting on in fact 6.00pm we found her picked her and marked her with red (we use red because we find it easiest to see in large colonies) waited a while for the paint to dry then placed her back on the same frame that she had come from, a few workers came to her and tried to clean of the paint but were unable to do so then they turned on her biting her legs and wings and then balling her, we were able to get her out and had to place her in a cage, we then placed her back in the hive between the frames and left her for three days she was allowed back in to the colony but the are now producing queen cells so they must not like her and think there is something wrong with her, we will now only mark between 11am and 3pm when the older bees are out foraging. Chris