A test frame will probably do nothing other than tell you if there is a queen as your bees will be too old to raise any descent Qcs.
Sad to say but you don't have any options with overwintered bees like that, especially as you don't have any other hives to combine with. A mated queen will be wasted money.
Sorry to hear you have lost all your hives, 4/4 is a kick in the teeth and I would definitely look for lessons learnt to make sure it doesn't happen again.Size of hives going into winter (combine in the autumn to only overwinter strong hives), varroa treatment(as it has been said above treat autumn, winter, and spring), queen strength, etc.