It applies at each move!
JBM is correct, you want all or most of the bees from the original site to be on their last legs before moving the second time. could be 4-6 weeks.
I move hives to my dad's back garden 26 miles away! Just because it's secure (and close to work so not a great problem).
However, as the old man is 85 (and self isolating) the nuc that was dropped off 3 weeks ago will have to do whatever mother nature intends!
I expect there to be a couple of swarms on the loose before I'm able to visit!