one thing is the acceptance and the other thing is the survivorship after introduction
less the bees will accept more chances of acceptance , bees pass into emergency Qless situation in first 24-36h so introduction need to be immediately and also bees ussually accept/reject un introduced Q in first 24 h(whatever introduced method) but that do no means that they may not supercedure her after , so
the introduced Q is better if she is on her 4th week(21-28 days old better pheromones,ovariole,spermatotheque.......) well mated non stopp laying and also if have close kinship relationship(half sisters - daugther) so as bees recognise her retinue pheromones and she is as good as pervious and healthy ,do no carry any diseases or has suffered an injury..
this year had a supercedure Q with her mother present(old enouf) and after while with 2 supercedure cells present plus the mother so that made me wonder for this daugter and decide test her, cut a nuc from another colony(good Q-half sisters) with emerging brood 1 store and 1 empty drawn comb and introduce her straight in that comb , bees accept her but supercedure after so was right wonder for her cause seems she was not mated well and was not as good as nuc bees previous Q
ussually i do this when want test Q or when want supercedure a nuc Q not mated well or lost in mate fly and do no ve any Qcell available for bigger colonies better follow safer methods (Qcell or 2 days cage? making em hopelessly Qless not emergency ?)
