You will need a National or second commercial brood chamber using this method... Once the colony is doing well - say well into April, you can set up the hive as follows, from the bottom up.
Floor with entrance closed. National brood box with the national frames and no queen. Queen excluder. Eke (spacer of, say 1" wood) with an entrance to allow the bees in and out. Commercial brood box with the queen and frames pushed together and extra frames/foundation added to the sides. Crown board. Roof.
Bees will cover the brood that's in the lower box so you don't lose the brood. After 3 weeks - or 24 days if there is drone brood - all the brood will have emerged. During these 3 weeks, the bees will use the top entrance and as any stores are below them, they will (should) also remove the stores from the National frames. Once the nat frames have no brood in them, they can be removed with the brood box they are in. The eke and excluder can be removed and the colony can return to using the lower entrance. If there is not much forage, you could give them some thin syrup to help them draw the new foundation you have added to the box.
If you are not sure where the queen is, you could separate the frames into two boxes and after a few days (3+) you will see, by the presence of eggs, which box the queen is in. You still have to find her but it will be a little easier!