JEP
New Bee
OK they are your bees so go for it. It doesn't matter what time of day you put the frames in the nuc box - I would suggest sometime when it is light so that you can see what you are doing but it is up to you. After you transfer a frame with some eggs you might want to try to include some with capped brood so that the bees don't have to look after too many larvae. I would also include some frames with honey but again up to you. It is common practice to shake the bees from a few other combs in as well to ensure that there are enough bees to prevent brood chill. It would be a good idea to ventilate the box they travel in with a screen and I would open the entrance or transfer them to a full hive straight away on arrival at the new site. Good luck!