To be honest , I couldn't tell you, and to be doubly honest, i really have never even considered that they use it, instead just build off of it! I know bees can re use wax but i was under the impression that this was uneconomical for bees to do. It was my way of thinking that they always built on it, and the bottom of each hexagonal became automatically the bottom of each cell? When ever i have scraped damaged cells by either wax moth or mice, or removed a little honey from frames to make them useable for swarm traps i have always scraped back to the foundation, so i would assume that means they have built on i ?
I believe your in to "foundation less" so you would know more Tom?
Interesting, please tell more?