Does the Q shake off first or last

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Location
Traditional Surrey
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I am having a dreadful start to the season, partly courtesy of the dreaded mite but mainly PPB as usual and feel like I have forgotten what little I know. So, transferring a test frame without nurse bees, look for Q, three good shakes and a look to check the Q is not among the few bees remaining. Is there ANY chance she went over? Does she come off after the nurse bees or before?
 
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I am having a dreadful start to the season, partly courtesy of the dreaded mite but mainly PPB as usual and feel like I have forgotten what little I know. So, transferring a test frame without nurse bees, look for Q, three good shakes and a look to check the Q is not among the few bees remaining. Is there ANY chance she went over? Does she come off after the nurse bees or before?

I wouldn't shake a frame without first making sure the queen wasn't on, unless it wasn't a concern she was shaken off. I always find and cage queen before shaking frames or doing any rough manipulations. Find the queen first before transfering frames
 
thinking about the physics, a queen weighs more and has longer legs than a worker, so it's quite likely that the momentum of a shake will throw the queen off at least as easily as any worker.
 
Just shaken a frame full of bees with a queen walking around on it. She was heavily clipped so I thought it would be easier to shake her ontop of the remaining frames.
First 2 gentle shakes, she was still attached, a more vigorous shake, she was one of the remaining 20 or so bees still attached.
Decided to let her crawl on my hand and placed her on the top bars.
So this queen didn't want to let go.
 

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