G'day Gary.
If in doubt, send a sample to Archie Murchie or Ivan Forsythe in the AFBI laboratory or request an inspection via Tom Williamson, the Senior Bee Inspector.
A few questions for you:
You refer to a layer of dead bees on the floor of the hive. Define "layer". Was the entire floor completely covered?
On your repeat inspection, you note 20 to 30 dead bees. What is the timing between your inspections? Seven days after your last inspection, I would suggest that 20 dead bees is not a great amount.
Did you crush any bees when removing frames? This could easily account for the 20-30 dead bees you found.
A piece of advice. You comment that your bees do not like smoke. Try using a water mister/plant sprayer and spray the bees with the water mist rather than smoke. No additives needed!
I use a water spray when working with bees and I have lit my smoker the grand total of three times this year. Once it wasn't used, just "on hand", the second time was when I was checking an apiary when the oil seed rape was going over on a coolish evening. The third time was Saturday past when I lit my smoker for beginner beekeepers to use when I was assessing them for an apiary practical exam. When closing up the hives used, I employed a mist of water and the examinees who stayed back to help clear up the apiary were amazed at the way the bees cleared the top bars of the hives and stayed down in comparison to the situation when smoke was used.
What fuel are you using in your smoker and how much smoke are you using? Keep in mind that some colonies just do not like smoke.