Yes this method is used by Randy Oliver, but not to test for the presence of nosema but to determine whether the colony needs treatment. It is a form of sequential testing see his webpage for further details http://scientificbeekeeping.com/sick-bees-part-15-an-improved-method-for-nosema-sampling/
Well I have just made a slide from a blue crocus and measured the diameter of the pollen grains to find that it was 92 microns.
Shows you should beleive all you read!
I think you'll find that Bob talks about glycerin when he actually means glycerine jelly. Numount is used for slides of bee plants and you need to remove all water from the slide before using it.
Most people use Basic fuchsin in glyverine jelly for pollen, it is what Sawyer used. Safranine and...
I have a lumix TZ20 and I find that the range of focus for near shots is very limited. If the zoom is set too high the distance at which the camera will focus is too far. I would suggest that this is what your problem is.
Solutions are get closer and use less zoom
or get further away and use...
Field of vision, in my opinion, covers what I can see without moving the jar; i.e. 180 degrees, (or in a hex jar 3 adjoining sides. If it was a circular jar, you wouldn't be querying a label covering half the circumference. Same principle.
Would you really go to all that bother for Nosema, fumigation with 80% acetic acid for a fortnight will deal with nosema spores, now if your colony died out from something else.......
If the jar, instead of being presented angle forward was seen flat forward, one would be able to see three of the six faces in the one field of view.
From what the OP said this would show the weight label.
Depends on how you look at the jar, and if you look downwards at an angle you would also...
http://http://www.beekeepingforum.co.uk/album.php?albumid=496&pictureid=2642 and http://www.beekeepingforum.co.uk/album.php?albumid=496&pictureid=2641 and http://www.beekeepingforum.co.uk/picture.php?albumid=496&pictureid=2640
I wonder whether that is the case for AFB spores which can take a lot of heat before they are killed off the glass does get any hotter than 'hand hot' with two or three passes through the flame.
I have my reference slides mounted with Canada Balsam and a cover glass.
If I could post a photo onto this forum without having previously loaded it onto the web, I could show you a picture of a frame which at first glance shows a perfect brood pattern, on closer examination some (about 5 cell cappings) were discoloured.
This was not so obvious and easy to detect...
I don't think the usual microscopy course in UK handles the making of foul brood slides, because you need to be licenced to handle those bacteria and must do so in a bio-security location.