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Yes they do... and new ones are still being manufactured.

Have one that will extract 24 shallows or 12 deep frames, works really well ... bought from E H Thorne. One of our other extractors takes 30 deep frames or 30 shallows, but that is just radial.

Good girls.

Neglected, infertile, drone in worker cells, laying workers, buggered.

Nothing with bees is foolproof, you can have laying workers after two days with some, and you can have laying workers with a virgin queen present.

Depends on whether you carry out instrumental insemination? can be much later.

Laying workers.

When cleaning the tips I use a vacuum pump to first flush the tip through with washing up liquid, then bleach, then boiling water, followed by ethanol, then the tips put in autoclave and then when using flushed through with sterile saline. The saline driver is filled from a new sterile...

Buy some autoclave pouches to put your II tips and other suitable items in for sterilization. Similar to these....https://www.ebay.co.uk/itm/Self-Seal-Sterilisation-Pouches-90-x-260mm-Autoclave-200/153437321768?hash=item23b993ba28:g:VsEAAOSwcNFcoh7W

In hand.

Read this this thread on Beesource, it is long, but very informative, you will find out how to make or where to buy everything needed to do so. https://www.beesource.com/forums/showthread.php?263523-Instrumental-Insemination-Equipment

Best to put her in a nicot cage with a few young attendants and food, they don't keep well in an incubator, don't put much liquid in the cage or she will end up in a sticky mess.

Laying workers... and virgin queens cannot normally get through a queen excluder.

Seem to get roped into these things as well, had an afternoon on queen rearing, etc, for local bka a few days ago, also hired the village hall for them to have tea and cake afterwards.

My favorite method, lost one out of 190 last season, no direct intro losses so far this season... better success rate than caging the queens.

Move them three miles or more .

There are a few quite large forests here which are predominantly mature sweet chestnut trees, planted by the big estates as an alternative to the oak trees they cut down by the thousand.

Just started flowering here over the last few days, lots of honey bees working on them along with all the other things in flower now... the sweet chestnut will also be in flower very soon now, always do well from them.

Thought it was an excellent post.

They don't have any dysentery/poop problems with Nosema Ceranae, they have a lack of older foragers to help the colony build up, if a queen gets it then it affects her ovaries and egg laying.