polymath

Enrico i also may be switching soon so will come back to you.

The orignal paper by LASI where they treated twice not once.

however some research has found negative effects.

This paper covers the broodless periods

Here is research on multiple uses:

This article might interest a few in particular the table on different methods:

Sorry you are right 2.25 but as for more than one treatment you are wrong, and i justify that statement by an email from the researcher...

Generally mid december has been identified as when there is least brood - LASI research. Two treatments 5 - 10 day apart for maximum...